Soldatou, S., Eldjárn, G. H., Ramsay, A. , Van Der Hooft, J. J.J. , Hughes, A. H., Rogers, S. and Duncan, K. R. (2021) Comparative metabologenomics analysis of polar actinomycetes. Marine Drugs, 19(2), 103. (doi: 10.3390/md19020103) (PMID:33578887) (PMCID:PMC7916644)
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Publisher's URL: https://www.mdpi.com/1660-3397/19/2/103
Abstract
Biosynthetic and chemical datasets are the two major pillars for microbial drug discovery in the omics era. Despite the advancement of analysis tools and platforms for multi-strain metabolomics and genomics, linking these information sources remains a considerable bottleneck in strain prioritisation and natural product discovery. In this study, molecular networking of the 100 metabolite extracts derived from applying the OSMAC approach to 25 Polar bacterial strains, showed growth media specificity and potential chemical novelty was suggested. Moreover, the metabolite extracts were screened for antibacterial activity and promising selective bioactivity against drug-persistent pathogens such as Klebsiella pneumoniae and Acinetobacter baumannii was observed. Genome sequencing data were combined with metabolomics experiments in the recently developed computational approach, NPLinker, which was used to link BGC and molecular features to prioritise strains for further investigation based on biosynthetic and chemical information. Herein, we putatively identified the known metabolites ectoine and chrloramphenicol which, through NPLinker, were linked to their associated BGCs. The metabologenomics approach followed in this study can potentially be applied to any large microbial datasets for accelerating the discovery of new (bioactive) specialised metabolites.
| Item Type: | Articles |
|---|---|
| Additional Information: | This research was funded by Carnegie Trust Collaborative Research Grant (KRD, SR, SS). AR, KRD and SR were supported by the Biotechnology and Biological Sciences Research Council (BB/R022054/1). Additionally, genome sequencing was provided by MicrobesNG (http://www.microbesng.uk) which was supported by the Biotechnology and Biological Sciences Research Council (BB/L024209/1). |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Rogers, Dr Simon and Van Der Hooft, Mr Justin and Ramsay, Mr Andrew |
| Creator Roles: | Ramsay, A.Writing – review and editing Van Der Hooft, J. J.J.Writing – review and editing Rogers, S.Conceptualization, Methodology, Formal analysis, Investigation, Writing – original draft, Writing – review and editing, Supervision, Project administration, Funding acquisition |
| Authors: | Soldatou, S., Eldjárn, G. H., Ramsay, A., Van Der Hooft, J. J.J., Hughes, A. H., Rogers, S., and Duncan, K. R. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Science and Engineering > School of Computing Science |
| Journal Name: | Marine Drugs |
| Publisher: | MDPI |
| ISSN: | 1660-3397 |
| ISSN (Online): | 1660-3397 |
| Copyright Holders: | Copyright © 2021 The Authors |
| First Published: | First published in Marine Drugs 19(2):103 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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