Bandpass sorting of heterogeneous cells using a single surface acoustic wave transducer pair

Simon, G., Busch, C., Andrade, M. A. B., Reboud, J. , Cooper, J. M. , Desmulliez, M. P. Y., Riehle, M. O. and Bernassau, A. L. (2021) Bandpass sorting of heterogeneous cells using a single surface acoustic wave transducer pair. Biomicrofluidics, 15(1), 014105. (doi: 10.1063/5.0040181) (PMID:33537112) (PMCID:PMC7843154)

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Separation and sorting of biological entities (viruses, bacteria, and cells) is a critical step in any microfluidic lab-on-a-chip device. Acoustofluidics platforms have demonstrated their ability to use physical characteristics of cells to perform label-free separation. Bandpass-type sorting methods of medium-sized entities from a mixture have been presented using acoustic techniques; however, they require multiple transducers, lack support for various target populations, can be sensitive to flow variations, or have not been verified for continuous flow sorting of biological cells. To our knowledge, this paper presents the first acoustic bandpass method that overcomes all these limitations and presents an inherently reconfigurable technique with a single transducer pair for stable continuous flow sorting of blood cells. The sorting method is first demonstrated for polystyrene particles of sizes 6, 10, and 14.5 μm in diameter with measured purity and efficiency coefficients above 75 ± 6% and 85 ± 9%, respectively. The sorting strategy was further validated in the separation of red blood cells from white blood cells and 1 μm polystyrene particles with 78 ± 8% efficiency and 74 ± 6% purity, respectively, at a flow rate of at least 1 μl/min, enabling to process finger prick blood samples within minutes.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Riehle, Dr Mathis and Busch, Caroline and Cooper, Professor Jonathan and Reboud, Professor Julien
Authors: Simon, G., Busch, C., Andrade, M. A. B., Reboud, J., Cooper, J. M., Desmulliez, M. P. Y., Riehle, M. O., and Bernassau, A. L.
College/School:College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biomicrofluidics
Publisher:American Institute of Physics
ISSN (Online):1932-1058
Published Online:27 January 2021
Copyright Holders:Copyright © 2021 The Authors
First Published:First published in Biomicrofluidics 15(1): 014105
Publisher Policy:Reproduced under a Creative Commons License

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