The influence of apical periodontitis on the concentration of inflammatory mediators in peripheral blood plasma and the metagenomic profiling of endodontic infections: Study design and protocol

Georgiou, A.C., Crielaard, W., Ouwerling, P., McLean, W. , Lappin, D. F. and van der Waal, S.V. (2021) The influence of apical periodontitis on the concentration of inflammatory mediators in peripheral blood plasma and the metagenomic profiling of endodontic infections: Study design and protocol. Contemporary Clinical Trials Communications, 21, 100686. (doi: 10.1016/j.conctc.2020.100686) (PMID:33490705) (PMCID:PMC7810621)

Abstract

Increased systemic inflammation has been identified in presence of oral disease, specifically endodontic disease. It is important to investigate whether treatment of the oral disease ameliorates systemic inflammation. Furthermore, there is no information about the extent to which different microorganisms may trigger inflammatory response. Objectives: Primarily (i) to compare the plasma concentrations of inflammatory mediators of apical periodontitis (AP) subjects to controls, (ii) to evaluate whether elimination of the endodontic infection reduces systemic inflammation (iii) to investigate the microbiome of root canal infections. Secondarily i) to correlate the inflammatory mediator data with the microbiome data to investigate whether the type of infection influences the type and severity of the inflammatory condition ii) to examine patterns in the inflammatory mediator data before and after tooth extraction in order to establish a biomarker signature of AP/oral disease. This is a multi-centre prospective case-control intervention study. The cohort will consist of 30 healthy human volunteers with one or two teeth with a root-tip inflammation and 30 matched healthy controls. Peripheral blood will be drawn at 6 time points, 3 before and 3 after the extraction of the tooth with apical periodontitis. The teeth will be pulverized, DNA extraction and sequencing will be performed. This study aims to compare the concentration of inflammatory blood plasma proteins in between AP-subjects and controls at different time points before and after the tooth extraction in a systematic and complete way. Additionally the composition of the root canal microbiome in association with the inflammatory response of the host will be assessed.

• Item Type: Articles
• Additional Information: This study is supported by the department of Preventive Dentistry of the Academic Centre of Dentistry Amsterdam (ACTA) and the execution of this protocol will be supported by the annual research grant of the European Society of Endodontology of 2019 (ESE).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mclean, Professor William and Lappin, Dr David
• Authors: Georgiou, A.C., Crielaard, W., Ouwerling, P., McLean, W., Lappin, D. F., and van der Waal, S.V.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
• Journal Name: Contemporary Clinical Trials Communications
• Publisher: Elsevier
• ISSN: 2451-8654
• ISSN (Online): 2451-8654
• Published Online: 05 December 2020
• Copyright Holders: Copyright © 2020 The Authors
• First Published: First published in Contemporary Clinical Trials Communications 21:100686
• Publisher Policy: Reproduced under a Creative Commons License