GADD34 gene restores virulence in viral vector used in experimental stroke study

McCabe, C. , White, F., Brown, S.M. and Macrae, I.M. (2008) GADD34 gene restores virulence in viral vector used in experimental stroke study. Journal of Cerebral Blood Flow and Metabolism, 28(4), pp. 747-751. (doi: 10.1038/sj.jcbfm.9600565)

Abstract

GADD34 is expressed in the ischaemic brain and reverses protein synthesis shutdown. Consequently, GADD34 could have neuroprotective potential in stroke. BHK medium, a replication-deficient HSV viral vector (HSV1716) with no insert or containing full-length GADD34, the N terminal or a conserved portion of the gene, was injected into mouse brain before stroke. Infarct size was 1.0±0.26, 1.19±0.36, 1.5±0.36, 1.3±0.36, and 1.1±0.28 mm3, respectively. The increase in infarct size with full-length GADD34 was statistically significant (P<0.05). Immunohistochemistry confirmed viral protein expression. Tissue culture studies revealed GADD34 gene restored virulence in HSV1716, suggesting that HSV virulence, rather than increased GADD34, exacerbated ischaemic damage.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Macrae, Professor Mhairi and McCabe, Dr Chris
• Authors: McCabe, C., White, F., Brown, S.M., and Macrae, I.M.
• Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
• College/School: College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience
• Journal Name: Journal of Cerebral Blood Flow and Metabolism
• Publisher: Nature Publishing Group
• ISSN: 0271-678X
• ISSN (Online): 1559-7016
• Published Online: 10 October 2007
• Copyright Holders: Copyright © 2011 International Society for Cerebral Blood Flow and Metabolism
• First Published: First published in Journal of Cerebral Blood Flow and Metabolism 28(4):747-751
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher