Kennedy, S. , Miller, A. M., Wadsworth, R. M., McPhaden, A. R. and Wainwright, C. L. (2001) Validation of a technique to measure leukocyte adhesion to arterial segments: effects of drug treatments. Journal of Immunological Methods, 257(1-2), pp. 203-211. (doi: 10.1016/S0022-1759(01)00443-4) (PMID:11687253)
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Abstract
Adhesion and transmigration of leukocytes into arterial walls occurs after vascular injury and may play a role in the development of atherosclerosis and restenosis. This protocol presents a simple, rapid method for quantifying leukocyte adhesion to artery segments ex vivo. The procedure involves isolating leukocytes from rabbit whole blood and labelling with the gamma-emitting isotope 51Cr. Labelled leukocytes are added to open rings of subclavian artery taken from the same rabbit. After gamma counting, percentage leukocyte adhesion can be calculated with reference to a sample containing a quantity of labelled leukocytes equivalent to that which was added to the artery. Leukocyte adhesion was increased by l-NAME, thrombin and increasing incubation time and decreased by low temperatures. In addition, leukocyte adhesion was found to be increased following a vascular stretch injury performed in vitro. This protocol offers a number of advantages: the rapidity of the leukocyte isolation and labelling; the small quantity of leukocytes required; the ability to use autologous leukocytes; the applicability to whole arteries and arteries injured in vitro or in vivo, allowing the effects of vascular injury on leukocyte adhesion to be studied.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Miller, Dr Ashley and McPhaden, Dr Allan and Kennedy, Professor Simon |
| Authors: | Kennedy, S., Miller, A. M., Wadsworth, R. M., McPhaden, A. R., and Wainwright, C. L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Life Sciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
| Journal Name: | Journal of Immunological Methods |
| Publisher: | Elsevier |
| ISSN: | 0022-1759 |
| ISSN (Online): | 1872-7905 |
| Published Online: | 29 October 2001 |
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