Adhesion of Escherichia coli to nanostructured surfaces and the role of type 1 fimbriae

Kallas, P., Haugen, H. J., Gadegaard, N. , Stormonth-Darling, J. , Hulander, M., Andersson, M. and Valen, H. (2020) Adhesion of Escherichia coli to nanostructured surfaces and the role of type 1 fimbriae. Nanomaterials, 10(11), 2247. (doi: 10.3390/nano10112247) (PMID:33198386) (PMCID:PMC7696039)

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Abstract

Bacterial fimbriae are an important virulence factor mediating adhesion to both biotic and abiotic surfaces and facilitating biofilm formation. The expression of type 1 fimbriae of Escherichia coli is a key virulence factor for urinary tract infections and catheter-associated urinary tract infections, which represent the most common nosocomial infections. New strategies to reduce adhesion of bacteria to surfaces is therefore warranted. The aim of the present study was to investigate how surfaces with different nanotopography-influenced fimbriae-mediated adhesion. Surfaces with three different nanopattern surface coverages made in polycarbonate were fabricated by injection molding from electron beam lithography nanopatterned templates. The surfaces were constructed with features of approximately 40 nm width and 25 nm height with 100 nm, 250 nm, and 500 nm interspace distance, respectively. The role of fimbriae type 1-mediated adhesion was investigated using the E. coli wild type BW25113 and ΔfimA (with a knockout of major pilus protein FimA) and ΔfimH (with a knockout of minor protein FimH) mutants. For the surfaces with nanotopography, all strains adhered least to areas with the largest interpillar distance (500 nm). For the E. coli wild type, no difference in adhesion between surfaces without pillars and the largest interpillar distance was observed. For the deletion mutants, increased adhesion was observed for surfaces without pillars compared to surfaces with the largest interpillar distance. The presence of a fully functional type 1 fimbria decreased the bacterial adhesion to the nanopatterned surfaces in comparison to the mutants.

Item Type:Articles
Keywords:Nanostructured surface, injection molding, anti-adhesive, E. coli, fimbriae, anti-bacterial, biomaterial-associated infections (BAI).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stormonth-Darling, Mr John and Gadegaard, Professor Nikolaj
Creator Roles:
Gadegaard, N.Conceptualization, Methodology, Writing – review and editing
Stormonth-Darling, J.Formal analysis, Data curation, Investigation
Authors: Kallas, P., Haugen, H. J., Gadegaard, N., Stormonth-Darling, J., Hulander, M., Andersson, M., and Valen, H.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nanomaterials
Publisher:MDPI
ISSN:2079-4991
ISSN (Online):2079-4991
Published Online:12 November 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Nanomaterials 10(11): 2247
Publisher Policy:Reproduced under a Creative Commons License

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