Biophysical phenotyping of mesenchymal stem cells along the osteogenic differentiation pathway

Gavazzo, P., Viti, F., Donnelly, H., Oliva, M. A. G., Salmeron-Sanchez, M. , Dalby, M. J. and Vassalli, M. (2021) Biophysical phenotyping of mesenchymal stem cells along the osteogenic differentiation pathway. Cell Biology and Toxicology, (doi: 10.1007/s10565-020-09569-7) (PMID:33420657) (Early Online Publication)

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Abstract

Mesenchymal stem cells represent an important resource, for bone regenerative medicine and therapeutic applications. This review focuses on new advancements and biophysical tools which exploit different physical and chemical markers of mesenchymal stem cell populations, to finely characterize phenotype changes along their osteogenic differentiation process. Special attention is paid to recently developed label-free methods, which allow monitoring cell populations with minimal invasiveness. Among them, quantitative phase imaging, suitable for single-cell morphometric analysis, and nanoindentation, functional to cellular biomechanics investigation. Moreover, the pool of ion channels expressed in cells during differentiation is discussed, with particular interest for calcium homoeostasis.Altogether, a biophysical perspective of osteogenesis is proposed, offering a valuable tool for the assessment of the cell stage, but also suggesting potential physiological links between apparently independent phenomena.

Item Type:Articles
Status:Early Online Publication
Refereed:Yes
Glasgow Author(s) Enlighten ID:Salmeron-Sanchez, Professor Manuel and Vassalli, Dr Massimo and Donnelly, Miss Hannah and Dalby, Professor Matthew
Authors: Gavazzo, P., Viti, F., Donnelly, H., Oliva, M. A. G., Salmeron-Sanchez, M., Dalby, M. J., and Vassalli, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Cell Biology and Toxicology
Publisher:Springer
ISSN:0742-2091
ISSN (Online):1573-6822
Published Online:09 January 2021
Copyright Holders:Copyright © Springer Nature B.V. 2021
First Published:First published in Cell Biology and Toxicology 2021
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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