TopBP1 regulates human papillomavirus type 16 E2 interaction with chromatin

Donaldson, M.M. , Boner, W. and Morgan, I.M. (2007) TopBP1 regulates human papillomavirus type 16 E2 interaction with chromatin. Journal of Virology, 81(8), pp. 4338-4342. (doi: 10.1128/JVI.02353-06) (PMID:17287259)

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Human papillomavirus type 16 (HPV16) E2 regulates transcription from and replication of the viral genome, in association with viral and cellular factors. HPV16 E2 interacts functionally with TopBP1, a cellular protein essential for the initiation of cellular, and potentially viral, DNA replication. This report demonstrates that the absence of TopBP1 results in the redistribution of HPV16 E2 into an alternative cellular protein complex, resulting in enhanced affinity for chromatin. This redistribution does not significantly alter the ability of HPV16 E2 to either activate or repress transcription. We also show colocalization of both proteins on chromatin at late stages of mitosis, suggesting that TopBP1 could be the mitotic chromatin receptor for HPV16 E2. The possible significance of the results for the regulation of the viral life cycle is discussed.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Morgan, Professor Iain and Boner, Dr Winnie and Donaldson, Dr Mary
Authors: Donaldson, M.M., Boner, W., and Morgan, I.M.
Subjects:Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Journal of Virology
Journal Abbr.:J. Virol.
ISSN (Online):1098-5514

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