Camiolo, S. , Suárez, N. M., Chalka, A., Venturini, C., Breuer, J. and Davison, A. J. (2021) GRACy: a tool for analysing human cytomegalovirus sequence data. Virus Evolution, 7(1), veaa099. (doi: 10.1093/ve/veaa099) (PMID:33505707) (PMCID:PMC7816668)
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Abstract
Modern DNA sequencing has instituted a new era in human cytomegalovirus (HCMV) genomics. A key development has been the ability to determine the genome sequences of HCMV strains directly from clinical material. This involves the application of complex and often non-standardized bioinformatics approaches to analysing data of variable quality in a process that requires substantial manual intervention. To relieve this bottleneck, we have developed GRACy (Genome Reconstruction and Annotation of Cytomegalovirus), an easy-to-use tookit for analysing HCMV sequence data. GRACy automates and integrates modules for read filtering, genotyping, genome assembly, genome annotation, variant analysis and data submission. These modules were tested extensively on simulated and experimental data and outperformed generic approaches. GRACy is written in Python and is embedded in a graphical user interface with all required dependencies installed by a single command. It runs on the Linux operating system, and is designed to allow the future implementation of a cross-platform version. GRACy is distributed under a GPL 3.0 license and is freely available at https://bioinformatics.cvr.ac.uk/software/ with the manual and a test dataset.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Davison, Professor Andrew and Suarez, Dr Nicolas and Camiolo, Dr Salvatore |
| Authors: | Camiolo, S., Suárez, N. M., Chalka, A., Venturini, C., Breuer, J., and Davison, A. J. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Virus Evolution |
| Publisher: | Oxford University Press |
| ISSN: | 2057-1577 |
| ISSN (Online): | 2057-1577 |
| Published Online: | 30 December 2020 |
| Copyright Holders: | Copyright © 2020 The Authors |
| First Published: | First published in Virus Evolution 7(1): veaa099 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Related URLs: |
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