Integration of paper microfluidic sensors into contact lenses for tear fluid analysis

Moreddu, R., Elsherif, M., Adams, H., Moschou, D., Cordeiro, M. F., Wolffsohn, J. S., Vigolo, D., Butt, H., Cooper, J. M. and Yetisen, A. K. (2020) Integration of paper microfluidic sensors into contact lenses for tear fluid analysis. Lab on a Chip, 20(21), pp. 3970-3979. (doi: 10.1039/D0LC00438C) (PMID:32944726)

Abstract

In this article, using the integration of paper microfluidics within laser-inscribed commercial contact lenses, we demonstrate the multiplexed detection of clinically relevant analytes including hydrogen ions, proteins, glucose, nitrites and L-ascorbic acid, all sampled directly from model tears. In vitro measurements involved the optimization of colorimetric assays, with readouts collected, stored and analyzed using a bespoke Tears Diagnostics smartphone application prototype. We demonstrate the potential of the device to perform discrete measurements either for medical diagnosis or disease screening in the clinic or at the point-of-care (PoC), with future applications including monitoring of ocular infections, uveitis, diabetes, keratopathies and assessing oxidative stress.

• Item Type: Articles
• Additional Information: This research was supported by Engineering and Physical Sciences Research Council for (EPSRC) EP/T013567/1 grant.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cooper, Professor Jonathan
• Authors: Moreddu, R., Elsherif, M., Adams, H., Moschou, D., Cordeiro, M. F., Wolffsohn, J. S., Vigolo, D., Butt, H., Cooper, J. M., and Yetisen, A. K.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Lab on a Chip
• Publisher: Royal Society of Chemistry
• ISSN: 1473-0197
• ISSN (Online): 1473-0189
• Published Online: 04 September 2020