Evaluating the effects of SARS-CoV-2 Spike mutation D614G on transmissibility and pathogenicity

Volz, E. et al. (2021) Evaluating the effects of SARS-CoV-2 Spike mutation D614G on transmissibility and pathogenicity. Cell, 184(1), 64-75.e11. (doi: 10.1016/j.cell.2020.11.020) (PMID:33275900) (PMCID:PMC7674007)

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Abstract

Global dispersal and increasing frequency of the SARS-CoV-2 Spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of Spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large data set, well represented by both Spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the Spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant.

Item Type:Articles
Additional Information:EV acknowledges the MRC Centre for Global Infectious Disease Analysis MR/R015600/1. RJ and EV acknowledge funding from the European Commission (CoroNAb 101003653). VH was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) [grant number BB/M010996/1]. JTM, RMC, NJL and AR acknowledge the support of the Wellcome Trust (Collaborators Award 206298/Z/17/Z – ARTIC network). AR is supported by the European Research Council (grant agreement no. 725422 – ReservoirDOCS). DLR, ASF and ECT are supported by the MRC (MC_UU_1201412). JS was supported by the Biotechnology and Biological Sciences Research Council-funded South West Biosciences Doctoral Training Partnership [training grant reference BB/M009122/1]. TRC and NJL acknowledge support from the MRC which funded computational resources used by the project [grant reference MR/L015080/1]. TRC acknowledges funding as part of the BBSRC Institute Strategic Programme Microbes in the Food Chain BB/R012504/1 and its constituent projects BBS/E/F/000PR10348 and BBS/E/F/000PR10352]. AP and TRC acknowledge support from Supercomputing Wales, which is part-funded by the European Regional Development Fund (ERDF) via Welsh Government.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shah, Dr Rajiv and Li, Dr Kathy and Robertson, Professor David and Jesudason, Dr Natasha and Shepherd, Dr James and Da Silva Filipe, Dr Ana and Pascall, Dr David and Jarrett, Professor Ruth and Thomson, Professor Emma
Authors: Volz, E., Hill, V., McCrone, J. T., Price, A., Jorgensen, D., O'Toole, A., Southgate, J., Johnson, R., Jackson, B., Nascimento, F. F., Rey, S. M., Nicholls, S. M., Colquhoun, R. M., Da Silva Filipe, A., Shepherd, J., Pascall, D., Shah, R., Jesudason, N., Li, K., Jarrett, R., Pacchiarini, N., Bull, M., Geidelberg, L., Siveroni, I., CoG-UK Consortium, ., Goodfellow, I., Loman, N. J., Pybus, O. G., Robertson, D. L., Thomson, E. C., Rambaut, A., and Connor, T. R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Cell
Publisher:Elsevier (Cell Press)
ISSN:0092-8674
ISSN (Online):1097-4172
Published Online:19 November 2020
Copyright Holders:Copyright © 2020 The Author(s).
First Published:First published in Cell 184(1):64-75.e11
Publisher Policy:Reproduced under a Creative Commons license

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