Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures

Baka, R. et al. (2021) Quantitative proteomics of cerebrospinal fluid using tandem mass tags in dogs with recurrent epileptic seizures. Journal of Proteomics, 231, 103997. (doi: 10.1016/j.jprot.2020.103997) (PMID:33011347)

Abstract

This prospective study included four dog groups (group A: healthy dogs, groups B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), C: idiopathic epilepsy dogs without AEM administration, D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags approach using a Q-Exactive-Plus mass-spectrometer. Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P < 0.05). MMP2 and EFEMP2 appeared down-regulated whereas HP and APO-A1 were up-regulated (groups B, D). CLEC3B and PEBP4 were up-regulated whereas APO-A1 was down-regulated (group C). IGLL1 was down-regulated (groups B, C) and up-regulated (group D). EFEMP2 was the only protein detected among the four groups and PEBP4 was significantly different among the epileptic dogs. Western blot and SPARCL immunoassay were used to quantify HP abundance change, validating proteomic analysis. Both, showed good correlation with HP levels identified through proteomic analysis (r = 0.712 and r = 0.703, respectively).

• Item Type: Articles
• Additional Information: This research is co-financed by Greece and the European Union (European Social Fund- ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ). The proteomic analysis was supported by the European Commission FP7 “VetMedZg” project (grant number 621394) award to the Faculty of Veterinary Medicine, University of Zagreb, Croatia.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McLaughlin, Dr Mark and Eckersall, Professor David
• Authors: Baka, R., Eckersall, D., Horvatic, A., Gelemanovic, A., Mrljak, V., McLaughlin, M., Athanasiou, L. V., Papaioannou, N., Stylianaki, I., Hanh, H. Q., Chadwick, C. C., and Polizopoulou, Z.
• College/School: College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Proteomics
• Publisher: Elsevier
• ISSN: 1874-3919
• ISSN (Online): 1876-7737
• Published Online: 02 October 2020
• Copyright Holders: Copyright © 2020 Elsevier B.V.
• First Published: First published in Journal of Proteomics 231:103997
• Publisher Policy: Reproduced in accordance with the publisher copyright policy