Techniques for antifungal susceptibility testing of Candida albicans biofilms

Ramage, G. and López-Ribot, J. L. (2005) Techniques for antifungal susceptibility testing of Candida albicans biofilms. In: Ernst, E. J. and Rogers, P. D. (eds.) Antifungal Agents: Methods and Protocols. Series: Methods in molecular medicine, 118. Humana Press: Totowa, NJ, pp. 71-79. ISBN 9781592599431 (doi: 10.1385/1-59259-943-5:071)

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Abstract

Candida albicans is capable of forming biofilms on a variety of inert and biological surfaces. Cells in biofilms display phenotypic properties that are radically different from their free-floating planktonic counterparts, including their recalcitrance to antimicrobial agents. Consequently, Candida biofilm-associated infections are difficult to treat and to fully eradicate with standard antifungal therapy. Here, we describe a simple, fast, inexpensive and highly reproducible microtiter plate-based assay for the antifungal susceptibility testing of C. albicans biofilms. Because of its simplicity, compatibility with widely available 96-well microplate platform, high throughput, and automation potential, this assay represents an important tool towards the standardization of in vitro antifungal susceptibility testing of fungal biofilms.

Item Type:Book Sections
Additional Information:Print ISBN: 9781588292773
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon
Authors: Ramage, G., and López-Ribot, J. L.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Publisher:Humana Press
ISBN:9781592599431
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