Lyu, Y. , Yuan, X. , Glidle, A., Fu, Y., Furusho, H., Yang, T. and Yin, H. (2020) Automated Raman based cell sorting with 3D microfluidics. Lab on a Chip, 20(22), pp. 4235-4245. (doi: 10.1039/D0LC00679C) (PMID:33057530)
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Abstract
Raman activated cell sorting has emerged as a label-free technology that can link phenotypic function with genotypic properties of cells. However, its broad implementation is limited by challenges associated with throughput and the complexity of biological systems. Here, we describe a three-dimensional hydrodynamic focusing microfluidic system for a fully automated, continuous Raman activated cell sorting (3D-RACS). The system consists of a 3D printed detection chamber (1 mm3) that is integrated with a PDMS based sorting unit, optical sensors and an in-line collection module. It has the ability to precisely position cells in the detection chamber for Raman measurements, effectively eliminating spectroscopic interference from the device materials. This enables the sorting of a range of cell sizes (from 1 μm bacteria to 10's μm mammalian cells) with stable operation over >8 hours and high throughput. As a proof-of-concept demonstration, Raman-activated sorting of mixtures of Chlorella vulgaris and E. coli has demonstrated a purity level of 92.0% at a throughput of 310 cells per min. The platform employed in this demonstration features a simple “Raman window” detection system, enabling it to be built on a standard, inverted microscope. Together with its facile and robust operation, it provides a versatile tool for function-based flow cytometry and sorting applications in the fields of microbiology, biotechnology, life science and diagnostics.
Item Type: | Articles |
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Additional Information: | We thank support from both Nissan Chemical Ltd and the University of Glasgow for funding YL's studentship, Industrial Biotechnology Innovation Centre (IBioIC, Project-2016-152), and Natural Environment Research Council (NE/P003826/1). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Yin, Professor Huabing and Furusho, Dr Hitoshi and Yuan, Dr Xiaofei and Glidle, Dr Andrew and Lyu, Dr Yingkai and Fu, Yuchen |
Authors: | Lyu, Y., Yuan, X., Glidle, A., Fu, Y., Furusho, H., Yang, T., and Yin, H. |
College/School: | College of Science and Engineering College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Lab on a Chip |
Publisher: | Royal Society of Chemistry |
ISSN: | 1473-0197 |
ISSN (Online): | 1473-0189 |
Published Online: | 08 October 2020 |
Copyright Holders: | Copyright © 2020 The Royal Society of Chemistry |
First Published: | First published in Lab on a Chip 20(22):4235-4245 |
Publisher Policy: | Reproduced under a Creative Commons License |
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