Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites

Barker, A. R., Wickstead, B., Gluenz, E. and Gull, K. (2008) Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites. Molecular and Biochemical Parasitology, 162(2), pp. 112-122. (doi: 10.1016/j.molbiopara.2008.08.003) (PMID:18773926)

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Abstract

Trypanosoma brucei, the causative agent of African sleeping sickness, evades the immune response by expressing a coat of variant surface glycoprotein (VSG). VSG is expressed from a single telomeric expression site (ES), along with a number of expression site associated genes (ESAGs). Thus far, the function of most ESAGs is unknown. One ES contains the serum resistance associated gene (SRA), which confers resistance to trypanosome lytic factor in T. b. rhodesiense. Only three other ESAGs – 5, 6 and 7 – are present in this ES. ESAGs 6 and 7 encode a heterodimeric transferrin receptor, but the function of ESAG5 has not been identified. We present here a bioinformatic analysis of ESAG5 and distinguish between T. brucei-specific ESAGs and Genes Related to ESAG5 (GRESAGs), which occur outside of ESs in chromosomal-internal contexts. Further, a genome-wide survey of these genes across kinetoplastids identifies a family of GRESAG5s in a number of species. Analysis of phylogenetic relationships indicates that this family may have evolved from a single ancestral copy. Predicted properties of (GR)ESAG5 proteins indicate a glycosylated protein containing either a signal peptide or transmembrane domain. Further analysis indicates a possible relationship to the lipid transfer/lipopolysaccharide-binding family which includes the bactericidal/permeability increasing (BPI) protein. Together, these results provide insights into the structure and evolution of an important extended gene family, and present a number of testable hypotheses which will aid in elucidating the function of ESAG5.

Item Type:Articles
Additional Information:This work was supported by grants from the Wellcome Trust and EP Abraham Trust. AB holds a studentship from the Biotechnology and Biological Sciences Research Council (BBSRC). KG is a Wellcome Trust Principal Research Fellow.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gluenz, Dr Eva
Authors: Barker, A. R., Wickstead, B., Gluenz, E., and Gull, K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier
ISSN:0166-6851
ISSN (Online):1872-9428
Published Online:19 August 2008

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