Plasma pharmacokinetics of (poly)phenol metabolites and catabolites after ingestion of orange juice by endurance trained men

Pereira-Caro, G., Clifford, M. N., Polyviou, T., Ludwig, I. A., Alfheeaid, H. , Morena-Rojas, J. M., Garcia, A. L. , Malkova, D. and Crozier, A. (2020) Plasma pharmacokinetics of (poly)phenol metabolites and catabolites after ingestion of orange juice by endurance trained men. Free Radical Biology and Medicine, 160, pp. 784-795. (doi: 10.1016/j.freeradbiomed.2020.09.007) (PMID:32927016)

Abstract

The health benefits of orange juice (OJ) consumption are attributed in part to the circulating flavanone phase II metabolites and their microbial-derived ring fission phenolic catabolites. The present study investigated these compounds in the bloodstream after acute intake of 500 mL of OJ. Plasma samples obtained at 0, 1, 2, 3, 4, 5, 6, 7, 8 and 24 h after OJ intake were analysed by HPLC-HR-MS. Eleven flavanone metabolites and 36 phenolic catabolites were identified and quantified in plasma. The main metabolites were hesperetin-3′-sulfate with a peak plasma concentration (Cmax) of 80 nmol/L, followed by hesperetin-7-glucuronide (Cmax 24 nmol/L), hesperetin-3′-glucuronide (Cmax 18 nmol/L) and naringenin-7-glucuronide (Cmax 21 nmol/L). Among the main phenolic catabolites to increase in plasma after OJ consumption were 3′-methoxycinnamic acid-4′-sulfate (Cmax 19 nmol/L), 3-hydroxy-3-(3′-hydroxy-4′-methoxyphenyl)propanoic acid (Cmax 20 nmol/L), 3-(3′-hydroxy-4′-methoxyphenyl)propanoic acid (Cmax 19 nmol/L), 3-(4′-hydroxyphenyl)propanoic acid (Cmax 25 nmol/L), and 3-(phenyl)propanoic acid (Cmax 19 nmol/L), as well as substantial amounts of phenylacetic and hippuric acids. The comprehensive plasma pharmacokinetic profiles that were obtained are of value to the design of future ex vivo cell studies, aimed at elucidating the mechanisms underlying the potential health benefits of OJ consumption.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Malkova, Dr Dalia and Crozier, Professor Alan and Pereira Caro, Dr Gema and Garcia, Dr Ada and Alfheeaid, Dr Hani and Ludwig, Ms Iziar and Polyviou, Dr Thelma
• Authors: Pereira-Caro, G., Clifford, M. N., Polyviou, T., Ludwig, I. A., Alfheeaid, H., Morena-Rojas, J. M., Garcia, A. L., Malkova, D., and Crozier, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing; College of Science and Engineering > Scottish Universities Environmental Research Centre
• Journal Name: Free Radical Biology and Medicine
• Publisher: Elsevier
• ISSN: 0891-5849
• ISSN (Online): 1873-4596
• Published Online: 11 September 2020