Towards High-Throughput FLIM for Protein-Protein Interaction Screening of Live Cells and Tissue Microarrays

Barber, P.R. et al. (2008) Towards High-Throughput FLIM for Protein-Protein Interaction Screening of Live Cells and Tissue Microarrays. In: 2008 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro, Paris, France, 14-17 May 2008, pp. 356-359. ISBN 9781424420025 (doi: 10.1109/ISBI.2008.4541006)

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Abstract

Studying cellular protein-protein interactions in situ requires a technique such as fluorescence resonance energy transfer (FRET) which is sensitive on the nanometer scale. Observing FRET is significantly simplified if the fluorescence lifetime of the donor can be monitored. Results from live cells and tissue micro arrays are presented from an automated microscope incorporating time-domain TCSPC fluorescence lifetime imaging (FLIM). Novel hardware and software with a modular approach and scripting abilities allow us to work towards speed-optimized acquisition and ease of use to bring FLIM into the high-throughput regime.

Item Type:Conference Proceedings
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Carlin, Dr Leo
Authors: Barber, P.R., Pierce, G.P., Ameer-Beg, S.M., Matthews, D.R., Carlin, L.M., Keppler, M., Kelleher, M., Festy, F., Gillett, C., Springall, R., Ng, T.C., and Vojnovic, B.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
ISSN:1945-7928
ISBN:9781424420025
Published Online:13 June 2008

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