Matthews, D. R. et al. (2008) A High-Content Screening Platform Utilizing Polarization Anisotropy and FLIM Microscopy. In: SPIE BiOS 2008, San Jose, CA, USA, 21-23 Jan 2008, pp. 685919-1. ISBN 9780819470348 (doi: 10.1117/12.763310)
Full text not currently available from Enlighten.
Abstract
An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Förster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simultaneously capture the parallel and perpendicular components of both eGFP and mRFP1 fluorescence emission to provide a high-speed measurement of acceptor depolarization. Donor excited state lifetime measurements performed using laser scanning microscopy is then used to determine the FRET efficiency in a particular assay. A proof-of-principle assay is performed using mutant Jurkat human T-cells to illustrate the process by which FRET is first identified and then quantified by our high-content screening system.
| Item Type: | Conference Proceedings |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Carlin, Dr Leo |
| Authors: | Matthews, D. R., Ameer-Berg, S. M., Barber, P., Pierce, G. P., Newman, R. G., Vojnovic, B., Carlin, L. M., Keppler, M. D., Ng, T., Suhling, K., and Irving, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
| ISSN: | 1605-7422 |
| ISBN: | 9780819470348 |
| Published Online: | 05 March 2008 |
University Staff: Request a correction | Enlighten Editors: Update this record
Deposit and Record Details
Deposit and Record Details