Liao, Y. et al. (2016) The cardiomyocyte RNA-binding proteome: links to intermediary metabolism and heart disease. Cell Reports, 16(5), pp. 1456-1469. (doi: 10.1016/j.celrep.2016.06.084) (PMID:27452465) (PMCID:PMC4977271)
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Abstract
RNA functions through the dynamic formation of complexes with RNA-binding proteins (RBPs) in all clades of life. We determined the RBP repertoire of beating cardiomyocytic HL-1 cells by jointly employing two in vivo proteomic methods, mRNA interactome capture and RBDmap. Together, these yielded 1,148 RBPs, 391 of which are shared with all other available mammalian RBP repertoires, while 393 are thus far unique to cardiomyocytes. RBDmap further identified 568 regions of RNA contact within 368 RBPs. The cardiomyocyte mRNA interactome composition reflects their unique biology. Proteins with roles in cardiovascular physiology or disease, mitochondrial function, and intermediary metabolism are all highly represented. Notably, we identified 73 metabolic enzymes as RBPs. RNA-enzyme contacts frequently involve Rossmann fold domains with examples in evidence of both, mutual exclusivity of, or compatibility between RNA binding and enzymatic function. Our findings raise the prospect of previously hidden RNA-mediated regulatory interactions among cardiomyocyte gene expression, physiology, and metabolism.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Castello, Professor Alfredo |
Authors: | Liao, Y., Castello, A., Fischer, B., Leicht, S., Föehr, S., Frese, C. K., Ragan, C., Kurscheid, S., Pagler, E., Yang, H., Krijgsveld, J., Hentze, M. W., and Preiss, T. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
Journal Name: | Cell Reports |
Publisher: | Elsevier (Cell Press) |
ISSN: | 2211-1247 |
ISSN (Online): | 2211-1247 |
Published Online: | 21 July 2016 |
Copyright Holders: | Copyright © 2016 The Authors |
First Published: | First published in Cell Reports 16(5): 1456-1469 |
Publisher Policy: | Reproduced under a Creative Commons License |
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