The cardiomyocyte RNA-binding proteome: links to intermediary metabolism and heart disease

Liao, Y. et al. (2016) The cardiomyocyte RNA-binding proteome: links to intermediary metabolism and heart disease. Cell Reports, 16(5), pp. 1456-1469. (doi: 10.1016/j.celrep.2016.06.084) (PMID:27452465) (PMCID:PMC4977271)

[img]
Preview
Text
221913.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

4MB

Abstract

RNA functions through the dynamic formation of complexes with RNA-binding proteins (RBPs) in all clades of life. We determined the RBP repertoire of beating cardiomyocytic HL-1 cells by jointly employing two in vivo proteomic methods, mRNA interactome capture and RBDmap. Together, these yielded 1,148 RBPs, 391 of which are shared with all other available mammalian RBP repertoires, while 393 are thus far unique to cardiomyocytes. RBDmap further identified 568 regions of RNA contact within 368 RBPs. The cardiomyocyte mRNA interactome composition reflects their unique biology. Proteins with roles in cardiovascular physiology or disease, mitochondrial function, and intermediary metabolism are all highly represented. Notably, we identified 73 metabolic enzymes as RBPs. RNA-enzyme contacts frequently involve Rossmann fold domains with examples in evidence of both, mutual exclusivity of, or compatibility between RNA binding and enzymatic function. Our findings raise the prospect of previously hidden RNA-mediated regulatory interactions among cardiomyocyte gene expression, physiology, and metabolism.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Castello, Professor Alfredo
Authors: Liao, Y., Castello, A., Fischer, B., Leicht, S., Föehr, S., Frese, C. K., Ragan, C., Kurscheid, S., Pagler, E., Yang, H., Krijgsveld, J., Hentze, M. W., and Preiss, T.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Cell Reports
Publisher:Elsevier (Cell Press)
ISSN:2211-1247
ISSN (Online):2211-1247
Published Online:21 July 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Cell Reports 16(5): 1456-1469
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record