Running on empty: a metabolomics approach to investigating changing energy metabolism during fasted exercise and rest

Blackburn, G. et al. (2020) Running on empty: a metabolomics approach to investigating changing energy metabolism during fasted exercise and rest. Metabolites, 10(10), 399. (doi: 10.3390/metabo10100399) (PMID:33050077) (PMCID:PMC7600507)

[img] Text
221646.pdf - Published Version
Available under License Creative Commons Attribution.



Understanding the metabolic processes in energy metabolism, particularly during fasted exercise, is a growing area of research. Previous work has focused on measuring metabolites pre and post exercise. This can provide information about the final state of energy metabolism in the participants, but it does not show how these processes vary during the exercise and any subsequent post-exercise period. To address this, the work described here took fasted participants and subjected them to an exercise and rest protocol under laboratory settings, which allowed for breath and blood sampling both pre, during and post exercise. Analysis of the data produced from both the physiological measurements and the untargeted metabolomics measurements showed clear switching between glycolytic and ketolytic metabolism, with the liquid chromatography-mass spectrometry (LC-MS) data showing the separate stages of ketolytic metabolism, notably the transport, release and breakdown of long chain fatty acids. Several signals, putatively identified as short peptides, were observed to change in a pattern similar to that of the ketolytic metabolites. This work highlights the power of untargeted metabolomic methods as an investigative tool for exercise science, both to follow known processes in a more complete way and discover possible novel biomarkers.

Item Type:Articles
Additional Information:Funding: M.P.B. is funded by a Wellcome Trust core grant to the Wellcome Centre for Integrative Parasitology (grant 104111/Z/14/Z). Glasgow Polyomics is supported by the University of Glasgow and Wellcome Trust (Grant numbers 097821/Z/11/Z and 105614/Z/14/Z).
Glasgow Author(s) Enlighten ID:Gill, Professor Jason and Wilson, John and Achcar, Dr Fiona and Kerr, Mrs Erin and Blackburn, Dr Gavin and Burgess, Dr Karl and Barrett, Professor Michael
Authors: Blackburn, G., Hay, J., Skagen, C., Paul, E., Achcar, F., Wilson, J., Best, C., Manson, E., Burgess, K., Barrett, M. P., and Gill, J.
College/School:College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
College of Medical Veterinary and Life Sciences > School of Infection & Immunity
Journal Name:Metabolites
ISSN (Online):2218-1989
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Metabolites 10(10):399
Publisher Policy:Reproduced under a Creative Commons Licence

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
170547The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/ZRIII - Parasitology
190536Integrated Health - Polyomics and Systems Biomedicine (ISSF Bid)Anna DominiczakWellcome Trust (WELLCOTR)097821/Z/11/ZInstitute of Cardiovascular & Medical Sciences
172121Funding SchemesAnna DominiczakWellcome Trust (WELLCOTR)105614/Z/14/ZInstitute of Cardiovascular & Medical Sciences