MicroRNA‐17‐5p reduces inflammation and bone erosions in mice with collagen‐induced arthritis and directly targets the JAK/STAT pathway in rheumatoid arthritis fibroblast‐like synoviocytes

Najm, A. et al. (2020) MicroRNA‐17‐5p reduces inflammation and bone erosions in mice with collagen‐induced arthritis and directly targets the JAK/STAT pathway in rheumatoid arthritis fibroblast‐like synoviocytes. Arthritis and Rheumatology, 72(12), pp. 2030-2039. (doi: 10.1002/art.41441) (PMID:32683798)

Abstract

Objective: We undertook this study to examine microRNA (miRNA) expression across rheumatoid arthritis (RA) phenotypes, along with the effects and mechanisms of action of miRNA‐17‐5p (miR‐17). Methods: A miRNA array was performed in synovial tissue biopsied from patients with naive erosive RA (n = 3) and patients with nonerosive RA (n = 3). MicroRNA‐17 lipoplex was delivered intraarticularly in the murine collagen‐induced arthritis model. Clinical, histologic, and structural effects were studied over the course of arthritis. In‐depth studies of the mechanisms of action of miR‐17 were performed in primary RA fibroblast‐like synoviocytes (FLS) isolated from synovial tissue. Results: Fifty‐five miRNAs including miR‐17 were reduced in erosive RA. The miR‐17 transfection into arthritic paws reduced the clinical inflammation score between day 2 and day 7 (2.8 versus 1.9; P = 0.03). Synovial B cell, T cell, macrophage, and polynuclear neutrophil infiltration was significantly reduced. Structural damage was also decreased, as shown by a reduction in the number of osteoclasts detected using tartrate‐resistant acid phosphatase staining (osteoclast surface/bone surface 32% versus 18%; P = 0.005) and erosion score by computed tomography analysis (2.9 versus 1.7; P = 0.023). Proinflammatory cytokines from the interleukin‐6 (IL‐6) family and IL‐1β expression were also significantly reduced, but tumor necrosis factor was not. MicroRNA‐17 directly targeted the 3′‐untranslated regions of STAT3 and JAK1. STAT3 and JAK1 messenger RNA (mRNA) and protein expression were reduced in RA FLS following miR‐17 transfection. STAT3 and JAK1 mRNA and activation of STAT3, as assessed by immunohistochemistry, were also reduced in injected paws (% stained area 93% versus 62%; P = 0.035). Conclusion: We demonstrate an antiinflammatory and antierosive role of miR‐17 in vivo. This effect involves the suppression of the IL‐6 family autocrine‐amplifying loop through the direct targeting of JAK1 and STAT3.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Sood, Dr Shatakshi and Najm, Dr Aurelie and Goodyear, Professor Carl
• Authors: Najm, A., Masson, F.‐M., Preuss, P., Georges, S., Ory, B., Quillard, T., Sood, S., Goodyear, C. S., Veale, D. J., Fearon, U., Le Goff, B., and Blanchard, F.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Research Centre: College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Immunobiology
• Journal Name: Arthritis and Rheumatology
• Publisher: Wiley
• ISSN: 2326-5191
• ISSN (Online): 2326-5205
• Published Online: 19 July 2020
• Copyright Holders: Copyright © 2020 The Authors
• First Published: First published in Arthritis and Rheumatology 72(12): 2030-2039
• Publisher Policy: Reproduced under a Creative Commons License