Subsurface carbon monoxide oxidation capacity revealed through genome-resolved metagenomics of a carboxydotroph

Mu, A., Thomas, B. C., Banfield, J. F. and Moreau, J. W. (2020) Subsurface carbon monoxide oxidation capacity revealed through genome-resolved metagenomics of a carboxydotroph. Environmental Microbiology Reports, 12(5), pp. 525-533. (doi: 10.1111/1758-2229.12868) (PMID:32633030)

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Microbial communities play important roles in the biogeochemical cycling of carbon in the Earth's deep subsurface. Previously, we demonstrated changes to the microbial community structure of a deep aquifer (1.4 km) receiving 150 tons of injected supercritical CO2 (scCO2) in a geosequestration experiment. The observed changes support a key role in the aquifer microbiome for the thermophilic CO‐utilising anaerobe Carboxydocella , which decreased in relative abundance post‐scCO2 injection. Here, we present results from more detailed metagenomic profiling of this experiment, with genome resolution of the native carboxydotrophic Carboxydocella . We demonstrate a switch in CO‐oxidation potential by Carboxydocella through analysis of its carbon monoxide dehydrogenase (CODH) gene before and after the geosequestration experiment. We discuss the potential impacts of scCO2 on subsurface flow of carbon and electrons from oxidation of the metabolic intermediate carbon monoxide (CO).

Item Type:Articles
Additional Information:The authors acknowledge funding to JWM from the Commonwealth of Australia and industry sponsors through the CO2CRC Program, and the Victorian Life Sciences Computation Initiative (grant number VR0032).
Glasgow Author(s) Enlighten ID:Moreau, Dr John
Authors: Mu, A., Thomas, B. C., Banfield, J. F., and Moreau, J. W.
College/School:College of Science and Engineering > School of Geographical and Earth Sciences > Earth Sciences
Journal Name:Environmental Microbiology Reports
ISSN (Online):1758-2229
Published Online:06 July 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Environmental Microbiology Reports 12(5): 525-533
Publisher Policy:Reproduced under a Creative Commons License

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