Nanopatterned titanium implants accelerate bone formation in vivo

Greer, A. et al. (2020) Nanopatterned titanium implants accelerate bone formation in vivo. ACS Applied Materials and Interfaces, 12(30), pp. 33541-33549. (doi: 10.1021/acsami.0c10273) (PMID:32633478) (PMCID:PMC7467557)

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Accelerated de novo formation of bone is a highly desirable aim of implants targeting musculoskeletal injuries. To date, this has primarily been addressed by biologic factors. However, there is an unmet need for robust, highly reproducible yet economic alternative strategies that strongly induce an osteogenic cell response. Here, we present a surface engineering method of translating bioactive nanopatterns from polymeric in vitro studies to clinically relevant material for orthopedics: three-dimensional, large area metal. We use a titanium-based sol–gel whereby metal implants can be engineered to induce osteoinduction both in vitro and in vivo. We show that controlled disordered nanotopographies presented as pillars with 15–25 nm height and 100 nm diameter on titanium dioxide effectively induce osteogenesis when seeded with STRO-1-enriched human skeletal stem cells in vivo subcutaneous implantation in mice. After 28 days, samples were retrieved, which showed a 20-fold increase in osteogenic gene induction of nanopatterned substrates, indicating that the sol–gel nanopatterning method offers a promising route for translation to future clinical orthopedic implants.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Dalby, Professor Matthew and MacLaren, Dr Ian and Burgess, Dr Karl and Greer, Dr Andrew and Gadegaard, Professor Nikolaj and Meek, Professor Dominic and Turner, Dr Lesley-Anne
Authors: Greer, A., Goriainov, V., Kanczler, J., Black, C., Turner, L.-A., Meek, R., Burgess, K., MacLaren, I., Dalby, M., Oreffo, R., and Gadegaard, N.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
College of Science and Engineering > School of Engineering > Biomedical Engineering
College of Science and Engineering > School of Physics and Astronomy
Journal Name:ACS Applied Materials and Interfaces
Publisher:American Chemical Society
ISSN (Online):1944-8252
Published Online:06 July 2020
Copyright Holders:Copyright © 2020 American Chemical Society
First Published:First published in ACS Applied Materials and Interfaces 12(30):33541-33549
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190408Bioactive orthopaedic implants using nanopatterned 3D materialsNikolaj GadegaardMedical Research Council (MRC)G1000842ENG - Biomedical Engineering
190602Development of nanopatterned substrates for the delivery of high quality stem cellsNikolaj GadegaardBiotechnology and Biological Sciences Research Council (BBSRC)BB/K011235/1ENG - Biomedical Engineering