Changes in gingival crevicular fluid matrix metalloproteinase-8 levels during periodontal treatment and maintenance

Kinane, D.F., Darby, I.B., Said, S., Luoto, H., Sorsa, T., Tikanoja, S. and Mantyla, P. (2003) Changes in gingival crevicular fluid matrix metalloproteinase-8 levels during periodontal treatment and maintenance. Journal of Periodontal Research, 38, pp. 400-404. (doi: 10.1034/j.1600-0765.2003.00663.x)

Abstract

Keywords: periodontal therapy; gingival crevicular fluid; matrix metalloproteinase-8 Objectives:  The aim of this study was to investigate the effect of scaling and root planing (SRP) and the maintenance phase of treatment on the gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) levels. Materials and methods:  Clinical measurements and GCF samples were taken from four sites in 20 adult periodontitis patients before and after SRP and during a 3-month maintenance phase of treatment. MMP-8 levels were measured from GCF samples by time-resolved immunofluorometric assay (IFMA) with monoclonal antibodies. Results:  SRP improved the clinical indices as would be predicted, 6.1 mm (SD = 1.4) at baseline compared with 4.3 mm (SD = 1.6) post-treatment (P < 0.001). Attachment level (AL) reduced but not significantly between these two visits 13.4 mm (SD = 2.4) compared with 12.8 mm (SD = 2.4) (P < 0.08) post therapy. GCF MMP-8 levels reduced after initial treatment from 33.8 μg/30 s sample to 23.5 μg/30 s, which just failed to reach statistical significance (P = 0.07). However, when MMP-8 levels were expressed as a concentration, the differences following initial therapy were significant (54.1 ng/μL at baseline compared with 34.2 ng/μL post treatment; P < 0.005). The difference, however, between the baseline MMP-8 levels (33.8 ng/30 s) and the final visit (16 ng/30 s) following maintenance was markedly significant (P < 0.001) for both absolute amounts and on a concentration basis. Conclusion:  In conclusion, clinical improvement following SRP was associated with significant reductions in MMP-8 levels. The GCF concentration of MMP-8 decreased after initial therapy but reduced even more dramatically (approximately 50%) following a 3-month period of maintenance (P > 0.001).

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: UNSPECIFIED
• Authors: Kinane, D.F., Darby, I.B., Said, S., Luoto, H., Sorsa, T., Tikanoja, S., and Mantyla, P.
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: Journal of Periodontal Research