Magnetically triggered release of entrapped bioactive proteins from thermally responsive polymer-coated iron oxide nanoparticles for stem-cell proliferation

Walker, M. , Will, I., Pratt, A., Chechik, V., Genever, P. and Ungar, D. (2020) Magnetically triggered release of entrapped bioactive proteins from thermally responsive polymer-coated iron oxide nanoparticles for stem-cell proliferation. ACS Applied Nano Materials, 3(6), pp. 5008-5013. (doi: 10.1021/acsanm.0c01167) (PMID:32626842) (PMCID:PMC7325428)

Abstract

Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below the polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically triggered release, upon which it could increase mesenchymal stem cell proliferation. Once the polymer transition will be chemically adjusted above body temperature, this system could be used for targeted cell stimulation in model animals and humans.

• Item Type: Articles
• Additional Information: The authors acknowledge the EPSRC (strategic studentship EP/M508196/1 and impact accelerator funding EP/K504014/1).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Walker, Dr Matthew
• Authors: Walker, M., Will, I., Pratt, A., Chechik, V., Genever, P., and Ungar, D.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: ACS Applied Nano Materials
• Publisher: American Chemical Society
• ISSN: 2574-0970
• ISSN (Online): 2574-0970
• Published Online: 15 May 2020
• Copyright Holders: Copyright © 2020 American Chemical Society
• First Published: First published in ACS Applied Nano Materials 3(6):5008–5013
• Publisher Policy: Reproduced under a Creative Commons licence