Mayer, C.-D., Giclais, S., Alsehly, F. and Hoppler, S. (2020) Diverse LEF/TCF expression in human colorectal cancer correlates with altered Wnt-regulated transcriptome in a meta-analysis of patient biopsies. Genes, 11(5), e538. (doi: 10.3390/genes11050538)
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Abstract
Aberrantly activated Wnt signaling causes cellular transformation that can lead to human colorectal cancer. Wnt signaling is mediated by Lymphoid Enhancer Factor/T-Cell Factor (LEF/TCF) DNA-binding factors. Here we investigate whether altered LEF/TCF expression is conserved in human colorectal tumor sample and may potentially be correlated with indicators of cancer progression. We carried out a meta-analysis of carefully selected publicly available gene expression data sets with paired tumor biopsy and adjacent matched normal tissues from colorectal cancer patients. Our meta-analysis confirms that among the four human LEF/TCF genes, LEF1 and TCF7 are preferentially expressed in tumor biopsies, while TCF7L2 and TCF7L1 in normal control tissue. We also confirm positive correlation of LEF1 and TCF7 expression with hallmarks of active Wnt signaling (i.e., AXIN2 and LGR5). We are able to correlate differential LEF/TCF gene expression with distinct transcriptomes associated with cell adhesion, extracellular matrix organization, and Wnt receptor feedback regulation. We demonstrate here in human colorectal tumor sample correlation of altered LEF/TCF gene expression with quantitatively and qualitatively different transcriptomes, suggesting LEF/TCF-specific transcriptional regulation of Wnt target genes relevant for cancer progression and survival. This bioinformatics analysis provides a foundation for future more detailed, functional, and molecular analyses aimed at dissecting such functional differences.
| Item Type: | Articles |
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| Additional Information: | Funding: CM acknowledges funding from the Scottish Government: Rural & Environment Science & Analytical Services. (RESAS). SH is a Royal Society/Leverhulme Trust Senior Research Fellow (SRF\R1\191017) and acknowledges research funding from the Biotechnology and Biological Sciences Research Council (BB/S018190/1, BB/M001695/1). |
| Keywords: | Wnt, colorectal cancer, TCF, LEF, transcriptome. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Mayer, Dr Claus-Dieter |
| Creator Roles: | |
| Authors: | Mayer, C.-D., Giclais, S., Alsehly, F., and Hoppler, S. |
| College/School: | College of Science and Engineering > School of Mathematics and Statistics |
| Journal Name: | Genes |
| Publisher: | MDPI |
| ISSN: | 2073-4425 |
| ISSN (Online): | 2073-4425 |
| Copyright Holders: | Copyright © 2020 by the authors |
| First Published: | First published in Genes 11(5):e538 |
| Publisher Policy: | Reproduced under a Creative Commons Licence |
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