Trujillo Munoz, S. , Gonzalez Garcia, C., Rico Tortosa, P., Reid, A., Windmill, J., Dalby, M. J. and Salmeron-Sanchez, M. (2020) Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors. Biomaterials, 252, 120104. (doi: 10.1016/j.biomaterials.2020.120104)
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Abstract
Extracellular matrix (ECM)-derived matrices such as Matrigel are used to culture numerous cell types in vitro as they recapitulate ECM properties that support cell growth, organisation, migration and differentiation. These ECM-derived matrices contain various growth factors which make them highly bioactive. However, they suffer lot-to-lot variability, undefined composition and lack of controlled physical properties. There is a need to develop rationally designed biomaterials that can also recapitulate ECM roles. Here, we report the development of Fibronectin (FN)-based 3D hydrogels of controlled stiffness and degradability that incorporate full-length FN to enable solid-phase presentation of growth factors in a physiological manner. We demonstrate, in vitro and in vivo, the effect of incorporating vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) in these hydrogels to enhance angiogenesis and bone regeneration, respectively. These hydrogels represent a step-change in the design of well-defined, reproducible, synthetic microenvironments for 3D cell culture that incorporate growth factors to achieve functional effects.
| Item Type: | Articles |
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| Additional Information: | This study was supported by the UK Regenerative Medicine Platform (MRC grant MR/L022710/1), the UK Engineering and Physical Sciences Research Council (EPSRC EP/P001114/1) and a programme grant from the Sir Bobby Charlton Foundation. μCT work was supported by the European Research Council (ERC) under the European Union's Seventh Framework Programme (FP7/2007-2013) (grant agreement No. [615030]). S.T. acknowledges support from the University of Glasgow through their internal scholarship funding program. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel and Windmill, Professor James and Trujillo Munoz, Dr Sara and Rico Tortosa, Dr Patricia and Dalby, Professor Matthew and Gonzalez Garcia, Dr Cristina |
| Creator Roles: | Trujillo Munoz, S.Conceptualization, Methodology, Investigation, Writing – original draft Dalby, M.Conceptualization, Methodology, Writing – original draft, Supervision Salmeron-Sanchez, M.Conceptualization, Methodology, Writing – original draft, Supervision, Funding acquisition Gonzalez Garcia, C.Methodology, Investigation Rico Tortosa, P.Methodology, Investigation Windmill, J.Methodology, Supervision |
| Authors: | Trujillo Munoz, S., Gonzalez Garcia, C., Rico Tortosa, P., Reid, A., Windmill, J., Dalby, M. J., and Salmeron-Sanchez, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Biomaterials |
| Publisher: | Elsevier |
| ISSN: | 0142-9612 |
| ISSN (Online): | 1878-5905 |
| Published Online: | 07 May 2020 |
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