Zhou, Y. , Zammit, P., Zickus, V., Taylor, J. M. and Harvey, A. R. (2020) Twin-Airy point-spread function for extended-volume particle localization. Physical Review Letters, 124(19), 198104. (doi: 10.1103/PhysRevLett.124.198104) (PMID:32469536)
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Abstract
The localization of point sources in optical microscopy enables nm-precision imaging of single-molecules and biological dynamics. We report a new method of localization microscopy using twin Airy beams that yields precise 3D localization with the key advantages of extended depth range, higher optical throughput, and potential for imaging higher emitter densities than are possible using other techniques. A precision of better than 30 nm was achieved over a depth range in excess of 7μm using a 60×, 1.4 NA objective. An illustrative application to extended-depth-range blood-flow imaging in a live zebrafish is also demonstrated.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Zickus, Dr Vytautas and Taylor, Dr Jonathan and Zhou, Mr Yongzhuang and Zammit, Mr Paul and Harvey, Professor Andy |
| Authors: | Zhou, Y., Zammit, P., Zickus, V., Taylor, J. M., and Harvey, A. R. |
| College/School: | College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Physical Review Letters |
| Publisher: | American Physical Society |
| ISSN: | 0031-9007 |
| ISSN (Online): | 1079-7114 |
| Copyright Holders: | Copyright © 2020 The Authors |
| First Published: | First published in Physical Review Letters 124(19):198104 |
| Publisher Policy: | Reproduced under a Creative Commons licence |
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