Zhou, Y. and Carles, G. (2020) Precise 3D particle localization over large axial ranges using secondary astigmatism. Optics Letters, 45(8), pp. 2466-2469. (doi: 10.1364/OL.388695) (PMID:32287260)
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Abstract
We propose an analytical pupil phase function employing cropped secondary astigmatism for extended-depth nanoscale 3D-localization microscopy. The function provides high localization precision in all three dimensions, which can be maintained over extended axial ranges, customizable up to two orders of magnitude relative to the conventional, diffraction-limited imaging. This enables, for example, capturing nanoscale dynamics within a whole cell. The flexibility and simplicity in the implementation of the proposed phase function make its adoption in localization-based microscopy attractive. We demonstrate and validate its application to real-time imaging of 3D fluid flow over a depth of 40 µm with a numerical aperture of 0.8.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Carles Santacana, Dr Guillem and Zhou, Mr Yongzhuang |
| Authors: | Zhou, Y., and Carles, G. |
| College/School: | College of Science and Engineering > School of Physics and Astronomy |
| Journal Name: | Optics Letters |
| Publisher: | Optical Society of America |
| ISSN: | 0146-9592 |
| ISSN (Online): | 1539-4794 |
| Copyright Holders: | Copyright © 2020 Optical Society of America |
| First Published: | First published in Optics Letters 45(8):2466-2469 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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