Mitochondrial genome engineering: the revolution may not be CRISPR-ized

Gammage, P. A. , Moraes, C. T. and Minczuk, M. (2018) Mitochondrial genome engineering: the revolution may not be CRISPR-ized. Trends in Genetics, 34(2), pp. 101-110. (doi: 10.1016/j.tig.2017.11.001) (PMID:29179920) (PMCID:PMC5783712)

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Engineering of mammalian mtDNA has been hampered by an inability to import nucleic acids into mitochondria. A limited toolkit exists for manipulation of mammalian mtDNA, relying on protein-only nucleolysis and heteroplasmy-shifting approaches. Although present in lower metazoans, the weight of evidence against an efficient endogenous RNA import mechanism in mammalian mitochondria is considerable. Controversially, the application of CRISPR/Cas9 for manipulation of mammalian mtDNA in human cells has been reported.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Gammage, Dr Payam
Authors: Gammage, P. A., Moraes, C. T., and Minczuk, M.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Trends in Genetics
ISSN (Online):1362-4555
Published Online:24 November 2017
Copyright Holders:Copyright © 2017 The Authors
First Published:First published in Trends in Genetics 34(2):101-110
Publisher Policy:Reproduced under a Creative Commons License

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