Gammage, P. A. , Moraes, C. T. and Minczuk, M. (2018) Mitochondrial genome engineering: the revolution may not be CRISPR-ized. Trends in Genetics, 34(2), pp. 101-110. (doi: 10.1016/j.tig.2017.11.001) (PMID:29179920) (PMCID:PMC5783712)
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Abstract
Engineering of mammalian mtDNA has been hampered by an inability to import nucleic acids into mitochondria. A limited toolkit exists for manipulation of mammalian mtDNA, relying on protein-only nucleolysis and heteroplasmy-shifting approaches. Although present in lower metazoans, the weight of evidence against an efficient endogenous RNA import mechanism in mammalian mitochondria is considerable. Controversially, the application of CRISPR/Cas9 for manipulation of mammalian mtDNA in human cells has been reported.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Gammage, Dr Payam |
Authors: | Gammage, P. A., Moraes, C. T., and Minczuk, M. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Trends in Genetics |
Publisher: | Elsevier |
ISSN: | 0168-9525 |
ISSN (Online): | 1362-4555 |
Published Online: | 24 November 2017 |
Copyright Holders: | Copyright © 2017 The Authors |
First Published: | First published in Trends in Genetics 34(2):101-110 |
Publisher Policy: | Reproduced under a Creative Commons License |
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