McConnell, G., Smith, G., Girkin, J., Gurney, A. and Ferguson, A. (2003) Two-photon microscopy of fura-2-loaded cardiac myocytes with an all-solid-state tunable and visible femtosecond laser source. Optics Letters, 28(19), pp. 1742-1744. (doi: 10.1364/OL.28.001742)
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Abstract
We report a robust and reliable platform source for visible-wavelength multiphoton microscopy that is based on nonlinear optical methods. We demonstrate a synchronously pumped, singly resonant optical parametric oscillator with simultaneous intracavity third-order quasi-phase matching in a single crystal that generates continuously tunable, visible, and femtosecond-pulsed radiation. The application of the system is demonstrated by two-photon laser-scanning fluorescence microscopy of rabbit cardiac myocytes loaded with the fluorescent Ca2+ indicator fura-2.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Smith, Professor Godfrey |
Authors: | McConnell, G., Smith, G., Girkin, J., Gurney, A., and Ferguson, A. |
College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
Journal Name: | Optics Letters |
Publisher: | Optical Society of America |
ISSN: | 0146-9592 |
ISSN (Online): | 1539-4794 |
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