Oo, S. L., Ilyas, A.-M., Karthikeyan, V. , Arava, C. M., Kong, E. Y., Yeung, C.-C., Chen, X., Yu, P. K.N. and Vellaisamy, R. A.L. (2020) Gating a single cell: a label-free and real-time measurement method for cellular progression. Analytical Chemistry, 92(2), pp. 1738-1745. (doi: 10.1021/acs.analchem.9b03136) (PMID:31904934)
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Abstract
There is an ever-growing need for more advanced methods to study the response of cancer cells to new therapies. To determine cancer cells’ response from a cell-mortality perspective to various cancer therapies, we report a label-free and real time method to monitor the in situ response of individual HeLa cells using a single cell gated transistor (SCGT). As a cell undergoes apoptotic cell death, it experiences changes in morphology and ion concentrations. This change is well in line with the threshold voltage of the SCGT, which has been verified by correlating the data with the cell morphologies by scanning electron microscopy and the ion-concentration analysis by inductively-coupled plasma mass spectrometry (ICPMS). This SCGT could replace patch clamps to study single cell activity via direct measurement in real time. Importantly, this SCGT can be used to study the electrical response of a single cell to stimuli that leaves the membrane intact.
Item Type: | Articles |
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Additional Information: | We acknowledge the grants from RGC of HKSAR project number T42-103/16N. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Vellaisamy, Professor Roy and Karthikeyan, Dr Vaithinathan |
Authors: | Oo, S. L., Ilyas, A.-M., Karthikeyan, V., Arava, C. M., Kong, E. Y., Yeung, C.-C., Chen, X., Yu, P. K.N., and Vellaisamy, R. A.L. |
College/School: | College of Science and Engineering > School of Engineering |
Journal Name: | Analytical Chemistry |
Publisher: | American Chemical Society |
ISSN: | 0003-2700 |
ISSN (Online): | 1520-6882 |
Published Online: | 06 January 2020 |
Copyright Holders: | Copyright © 2020 American Chemical Society |
First Published: | First published in Analytical Chemistry 92(2):1738-1745 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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