Visfatin mediates malignant behaviors through adipose-derived stem cells intermediary in breast cancer

Huang, J.-Y., Wang, Y.-Y., Lo, S. , Tseng, L.-M., Chen, D.-R., Wu, Y.-C., Hou, M.-F. and Yuan, S.-S. F. (2020) Visfatin mediates malignant behaviors through adipose-derived stem cells intermediary in breast cancer. Cancers, 12(1), 29. (doi: 10.3390/cancers12010029) (PMID:31861872) (PMCID:PMC7016886)

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Adipose-derived stem cells (ADSCs) have been implicated in tumor growth and metastasis in breast cancer. ADSCs exhibit tumor tropism, and are of increasing clinical relevance due to the autologous fat grafting for breast reconstruction. Although we have previously shown that a high level of the adipocytokine visfatin in human breast cancer tissues correlated with tumor progression mediated by cAbl and STAT3, the effects of visfatin in the tumor microenvironment are unclear. To understand how visfatin modulates breast cancer within the tumor-stromal environment, we examined determinants of breast cancer progression using a visfatin-primed ADSCs-tumor co-culture model. ADSCs were isolated from tumor-free adipose tissue adjacent to breast tumors. ADSCs were treated with or without visfatin for 48 h and then collected for co-culture with breast cancer cell line MDA-MB-231 for 72 h in a transwell system. We found that the MDA-MB-231 cells co-cultured with visfatin-treated ADSCs (vADSCs) had higher levels of cell viability, anchorage independent growth, migration, invasion, and tumorsphere formation than that co-cultured with untreated ADSCs (uADSCs). Growth differentiation factor 15 (GDF15) upregulation was found in the co-culture conditioned medium, with GDF15 neutralizing antibody blocking the promoting effect on MDA-MB-231 in co-culture. In addition, a GDF15-induced AKT pathway was found in MDA-MB-231 and treatment with PI3K/AKT inhibitor also reversed the promoting effect. In an orthotopic xenograft mouse model, MDA-MB-231 co-injected with vADSCs formed a larger tumor mass than with uADSCs. Positive correlations were noted between visfatin, GDF15, and phosphor-AKT expressions in human breast cancer specimens. In conclusion, visfatin activated GDF15-AKT pathway mediated via ADSCs to facilitate breast cancer progression.

Item Type:Articles
Keywords:Breast cancer, visfatin, adipose-derived stem cells (ADSCs), GDF15.
Glasgow Author(s) Enlighten ID:Lo, Professor Steven
Creator Roles:
Lo, S.Conceptualization, Writing – review and editing
Authors: Huang, J.-Y., Wang, Y.-Y., Lo, S., Tseng, L.-M., Chen, D.-R., Wu, Y.-C., Hou, M.-F., and Yuan, S.-S. F.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Cancers
ISSN (Online):2072-6694
Published Online:20 December 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Cancers 12(1): 29
Publisher Policy:Reproduced under a Creative Commons License

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