Sepp, K., Lee, M., Bluntzer, M., Helgason, G. V. , Hulme, A. N. and Brunton, V. G. (2020) Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells. Journal of Medicinal Chemistry, 63(5), pp. 2028-2034. (doi: 10.1021/acs.jmedchem.9b01546) (PMID:31829628) (PMCID:PMC7073915)
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Abstract
Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug dis-tribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and speci-ficity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, na-nomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Helgason, Professor Vignir |
Authors: | Sepp, K., Lee, M., Bluntzer, M., Helgason, G. V., Hulme, A. N., and Brunton, V. G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Journal of Medicinal Chemistry |
Publisher: | American Chemical Society |
ISSN: | 0022-2623 |
ISSN (Online): | 1520-4804 |
Published Online: | 12 December 2019 |
Copyright Holders: | Copyright © 2019 American Chemical Society |
First Published: | First published in Journal of Medicinal Chemistry 63(5):2028-2034 |
Publisher Policy: | Reproduced in accordance with the publisher copyright policy |
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