Isolation and differentiation of murine macrophages

Rios, F. J. , Touyz, R. M. and Montezano, A. C. (2017) Isolation and differentiation of murine macrophages. In: Touyz, R. M. and Shiffrin, E. L. (eds.) Hypertension: Methods and Protocols. Series: Methods in molecular biology. Humana Press, pp. 297-309. ISBN 9781493966233 (doi: 10.1007/978-1-4939-6625-7_23)

Abstract

Macrophages play a major role in inflammation, wound healing, and tissue repair. Infiltrated monocytes differentiate into different macrophage subtypes with protective or pathogenic activities in vascular lesions. In the heart and vascular tissues, pathological activation promotes cardiovascular inflammation and remodeling and there is increasing evidence that macrophages play important mechanisms in this environment. Primary murine macrophages can be obtained from: bone marrow by different treatments (granulocyte-macrophage colony-stimulating factor—GM-CSF, macrophage colony-stimulating factor—M-CSF or supernatant of murine fibroblast L929), peritoneal cavity (resident or thioglycolate elicit macrophages), from the lung (alveolar macrophages) or from adipose tissue. In this chapter we describe some protocols to obtain primary murine macrophages and how to identify a pure macrophage population or activation phenotypes using different markers.

• Item Type: Book Sections
• Status: Published
• Glasgow Author(s) Enlighten ID: Rios, Dr Francisco and Montezano, Dr Augusto and Touyz, Professor Rhian
• Authors: Rios, F. J., Touyz, R. M., and Montezano, A. C.
• College/School: College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health
• Publisher: Humana Press
• ISSN: 1064-3745
• ISBN: 9781493966233