Bartolozzi, A., Viti, F., De Stefano, S., Sbrana, F., Petecchia, L., Gavazzo, P. and Vassalli, M. (2020) Development of label-free biophysical markers in osteogenic maturation. Journal of the Mechanical Behavior of Biomedical Materials, 103, 103581. (doi: 10.1016/j.jmbbm.2019.103581)
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Abstract
The spatial and temporal changes of morphological and mechanical properties of living cells reflect complex functionally-associated processes. Monitoring these modifications could provide a direct information on the cellular functional state. Here we present an integrated biophysical approach to the quantification of the morphological and mechanical phenotype of single cells along a maturation pathway. Specifically, quantitative phase microscopy and single cell biomechanical testing were applied to the characterization of the maturation of human foetal osteoblasts, demonstrating the ability to identify effective label-free biomarkers along this fundamental biological process.
Item Type: | Articles |
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Additional Information: | This work was supported by the Ministry of Education, Universities and Research of Italy, in the context of the FIRB ‘Futuro in Ricerca 2013’ project [grant number RBFR13V4M2]. |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Vassalli, Professor Massimo |
Authors: | Bartolozzi, A., Viti, F., De Stefano, S., Sbrana, F., Petecchia, L., Gavazzo, P., and Vassalli, M. |
College/School: | College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Journal of the Mechanical Behavior of Biomedical Materials |
Publisher: | Elsevier |
ISSN: | 1751-6161 |
ISSN (Online): | 1878-0180 |
Published Online: | 05 December 2019 |
Copyright Holders: | Copyright © 2019 Elsevier Ltd. |
First Published: | First published in Journal of the Mechanical Behavior of Biomedical Materials 103:103581 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
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