Lack of evidence of rotavirus‐dependent molecular mimicry as a trigger of coeliac disease

Ziberna, F. et al. (2016) Lack of evidence of rotavirus‐dependent molecular mimicry as a trigger of coeliac disease. Clinical and Experimental Immunology, 186(3), pp. 356-363. (doi: 10.1111/cei.12855) (PMID:27548641)

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Abstract

New data suggest the involvement of rotavirus (RV) in triggering autoimmunity in coeliac disease (CD) by molecular mimicry between the human‐transglutaminase protein and the dodecapeptide (260‐271 aa) of the RV protein VP7 (pVP7). To assess the role of RV in the onset of CD, we measured anti‐pVP7 antibodies in the sera of children with CD and of control groups. We analysed serum samples of 118 biopsy‐proven CD patients and 46 patients with potential CD; 32 children with other gastrointestinal diseases; 107 no‐CD children and 107 blood donors. Using enzyme‐linked immunosorbent assay (ELISA) assay, we measured immunoglobulin (Ig)A–IgG antibodies against the synthetic peptides pVP7, the human transglutaminase‐derived peptide (476–487 aa) which shows a homology with VP7 protein and a control peptide. The triple‐layered RV particles (TLPs) containing the VP7 protein and the double‐layered RV‐particles (DLPs) lacking the VP7 protein were also used as antigens in ELISA assay. Antibody reactivity to the RV‐TLPs was positive in 22 of 118 (18%) CD patients and in both paediatric (17 of 107, 16%) and adult (29 of 107, 27%) control groups, without showing a statistically significant difference among them (P = 0·6, P = 0·1). Biopsy‐proven CD patients as well as the adult control group demonstrated a high positive antibody reactivity against both pVP7 (34 of 118, 29% CD patients; 66 of 107, 62% adult controls) and control synthetic peptides (35 of 118, 30% CD patients; 56 of 107, 52% adult controls), suggesting a non‐specific response against RV pVP7. We show that children with CD do not have higher immune reactivity to RV, thus questioning the molecular mimicry mechanism as a triggering factor of CD.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:De Lorenzo, Dr Giuditta
Authors: Ziberna, F., De Lorenzo, G., Schiavon, V., Arnoldi, F., Quaglia, S., De Leo, L., Vatta, S., Martelossi, S., Burrone, O.R., Ventura, A., and Not, T.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
Journal Name:Clinical and Experimental Immunology
Publisher:Wiley
ISSN:0009-9104
ISSN (Online):1365-2249
Published Online:22 August 2016

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