Experimental approaches for examining apicoplast biology

Biddau, M. , Ovciarikova, J. and Sheiner, L. (2020) Experimental approaches for examining apicoplast biology. In: Tonkin, C. J. (ed.) Toxoplasma gondii: Methods and Protocols. Series: Methods in molecular biology (2071). Humana Press: New York, NY, pp. 221-243. ISBN 9781493998562 (doi:10.1007/978-1-4939-9857-9_13)

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Genetic manipulation is a powerful tool to study gene function but identifying the direct and primary functional outcomes of any gene depletion is crucial for this strategy to be productive. This is a major challenge for the study of apicoplast biology, because, in the absence of an efficient isolation method, apicoplast functions must be assayed in the parasite. These assays should be performed dynamically from the time of gene depletion, and include standards and controls that separate direct from indirect phenotypes. Here, we describe a pipeline for apicoplast functional analysis and highlight relevant mutant T. gondii cell lines and apicoplast markers that are available in the field and that enhance the specificity of phenotype description.

Item Type:Book Sections
Keywords:Apicoplast, fluorescence, import, live imaging, organelle, plastid, redox, qPCR, qRT-PCR, roGFP.
Glasgow Author(s) Enlighten ID:Biddau, Dr Marco and Ovciarikova, Miss Jana and Sheiner, Dr Lilach
Authors: Biddau, M., Ovciarikova, J., and Sheiner, L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Publisher:Humana Press
Published Online:23 November 2019

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