Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein

May, G. , Faatz, E., Lucht, J.M., Haardt, M., Bolliger, M. and Bremer, E. (1989) Characterization of the osmoregulated Escherichia coli proU promoter and identification of ProV as a membrane-associated protein. Molecular Microbiology, 3(11), pp. 1521-1531. (doi:10.1111/j.1365-2958.1989.tb00138.x) (PMID:2515417)

Full text not currently available from Enlighten.

Abstract

The Escherichia coli proU operon encodes a high-affinity, binding-protein-dependent transport system for the osmoprotectant glycine betaine. Expression of proU is osmoregulated, and transcription of this operon is greatly increased in cells grown at high osmolarity. Characterization of the proU operon and its promoter provided results similar to those published elsewhere (Gowrishankar, 1989; Stirling et al., 1989). The previously identified proU601 mutation, which leads to increased proU expression both at low- and high osmolarity, is a G to A transition in the Pribnow box of the proU promoter, which increases the homology of the -10 region to the consensus sequence of E. coli promoters. Using an antiserum raised against a ProV-beta-galactosidase hybrid protein, we have identified ProV as a protein associated with the cytoplasmic membrane. This cellular location is consistent with its proposed role as the energy-coupling component of the ProU transport system.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:May, Dr Gerhard
Authors: May, G., Faatz, E., Lucht, J.M., Haardt, M., Bolliger, M., and Bremer, E.
Subjects:Q Science > QH Natural history > QH345 Biochemistry
Q Science > QH Natural history > QH426 Genetics
Q Science > QR Microbiology
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Molecular Microbiology
Publisher:Wiley
ISSN:0950-382X
ISSN (Online):1365-2958

University Staff: Request a correction | Enlighten Editors: Update this record