The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei

Walrad, P. B., Capewell, P. , Fenn, K. and Matthews, K. R. (2012) The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei. Nucleic Acids Research, 40(7), pp. 2869-2883. (doi: 10.1093/nar/gkr1106) (PMID:22140102) (PMCID:PMC3326296)

[img]
Preview
Text
205118.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

8MB

Abstract

Post-transcriptional gene regulation is essential to eukaryotic development. This is particularly emphasized in trypanosome parasites where genes are co-transcribed in polycistronic arrays but not necessarily co-regulated. The small CCCH protein, TbZFP3, has been identified as a trans-acting post-transcriptional regulator of Procyclin surface antigen expression in Trypanosoma brucei. To investigate the wider role of TbZFP3 in parasite transmission, a global analysis of associating transcripts was carried out. Examination of a subset of the selected transcripts revealed their increased abundance through mRNA stabilization upon TbZFP3 ectopic overexpression, dependent upon the integrity of the CCCH zinc finger domain. Reporter assays demonstrated that this regulation was mediated through 3′-UTR sequences for two target transcripts. Global developmental expression profiling of the cohort of TbZFP3-selected transcripts revealed their significant enrichment in transmissible stumpy forms of the parasite. This analysis of the specific mRNAs selected by the TbZFP3mRNP provides evidence for a developmental regulon with the potential to co-ordinate genes important in parasite transmission.

Item Type:Articles
Additional Information:Funding: Wellcome Trust (088293, Programme Grant to K.R.M.) (supporting P.B.W, P.C. and K.F.) and Wellcome Trust, The Centre for Immunity, Infection and Evolution (095831, strategic award).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Capewell, Dr Paul
Authors: Walrad, P. B., Capewell, P., Fenn, K., and Matthews, K. R.
College/School:College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Nucleic Acids Research
Publisher:Oxford University Press
ISSN:0305-1048
ISSN (Online):1362-4962
Published Online:01 December 2011
Copyright Holders:Copyright © 2011 The Authors
First Published:First published in Nucleic Acids Research 40(7): 2869-2883
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record