HISTONE DEACETYLASE 9 stimulates auxin-dependent thermomorphogenesis in Arabidopsis thaliana by mediating H2A.Z depletion

van der Woude, L. C. et al. (2019) HISTONE DEACETYLASE 9 stimulates auxin-dependent thermomorphogenesis in Arabidopsis thaliana by mediating H2A.Z depletion. Proceedings of the National Academy of Sciences of the United States of America, 116(50), pp. 25343-25354. (doi: 10.1073/pnas.1911694116) (PMID:31767749) (PMCID:PMC6911240)

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Abstract

Many plant species respond to unfavorable high ambient temperatures by adjusting their vegetative body plan to facilitate cooling. This process is known as thermomorphogenesis and is induced by the phytohormone auxin. Here, we demonstrate that the chromatin-modifying enzyme HISTONE DEACETYLASE 9 (HDA9) mediates thermomorphogenesis but does not interfere with hypocotyl elongation during shade avoidance. HDA9 is stabilized in response to high temperature and mediates histone deacetylation at the YUCCA8 locus, a rate-limiting enzyme in auxin biosynthesis, at warm temperatures. We show that HDA9 permits net eviction of the H2A.Z histone variant from nucleosomes associated with YUCCA8, allowing binding and transcriptional activation by PHYTOCHROME INTERACTING FACTOR 4, followed by auxin accumulation and thermomorphogenesis.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Patitaki, Ms Eirini and Perrella, Dr Giorgio and Kaiserli, Dr Eirini and Vellutini, Elisa
Authors: van der Woude, L. C., Perrella, G., Snoek, B. L., van Hoogdalem, M., Novák, O., van Verk, M. C., van Kooten, H. N., Zorn, L. E., Tonckens, R., Dongus, J. A., Praat, M., Stouten, E. A., Proveniers, M. C. G., Vellutini, E., Patitaki, E., Shapulatov, U., Kohlen, W., Balasubramanian, S., Ljung, K., van der Krol, A. R., Smeekens, S., Kaiserli, E., and van Zanten, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Proceedings of the National Academy of Sciences of the United States of America
Publisher:National Academy of Sciences
ISSN:0027-8424
ISSN (Online):1091-6490
Published Online:25 November 2019
Copyright Holders:Copyright © 2019 PNAS
First Published:First published in Proceedings of the National Academy of Sciences of the United States of America 116(50):25343-25354
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
171989Transcriptional regulation of plant growth in nuclear microdomainsEirini KaiserliBiotechnology and Biological Sciences Research Council (BBSRC)BB/M023079/1Institute of Molecular, Cell & Systems Biology