Gould, G. , Bowman, P. and Smith, G. (2019) Cardiac SNARE expression in health and disease. Frontiers in Endocrinology, 10, 881. (doi: 10.3389/fendo.2019.00881) (PMID:31920989) (PMCID:PMC6930865)
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Abstract
SNARE proteins are integral to intracellular vesicular trafficking, which in turn is the process underlying the regulated expression of substrate transporters such as the glucose transporter GLUT4 at the cell surface of insulin target tissues. Impaired insulin stimulated GLUT4 trafficking is associated with reduced cardiac function in many disease states, most notably diabetes. Despite this, our understanding of the expression and regulation of SNARE proteins in cardiac tissue and how these may change in diabetes is limited. Here we characterize the array of SNARE proteins expressed in cardiac tissue, and quantify the levels of expression of VAMP2, SNAP23, and Syntaxin4—key proteins involved in insulin-stimulated GLUT4 translocation. We examined SNARE protein levels in cardiac tissue from two rodent models of insulin resistance, db/db mice and high-fat fed mice, and show alterations in patterns of expression are evident. Such changes may have implications for cardiac function.
Item Type: | Articles |
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Additional Information: | This work was supported by a Ph.D. scholarship to PRTB (FS/14/61/31284) and project grants (PG/18/47/33822) from The British Heart Foundation to GG and GS, and Diabetes UK (15/0005246 and 13/0004696 to GG). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Smith, Professor Godfrey and Bowman, Mr Peter and Gould, Professor Gwyn |
Authors: | Gould, G., Bowman, P., and Smith, G. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Molecular Biosciences |
Journal Name: | Frontiers in Endocrinology |
Publisher: | Frontiers Media |
ISSN: | 1664-2392 |
ISSN (Online): | 1664-2392 |
Copyright Holders: | Copyright © 2019 Bowman, Smith and Gould |
First Published: | First published in Frontiers in Endocrinology 10:881 |
Publisher Policy: | Reproduced under a Creative Commons License |
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