Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men

Scott, C. L., Wright, P. B., Milling, S. W.F. and Mowat, A. M. (2016) Isolation and identification of conventional dendritic cell subsets from the intestine of mice and men. In: Segura, E. and Onai, N. (eds.) Dendritic Cell Protocols. Series: Methods in molecular biology (1423). Humana Press: New York, pp. 101-118. ISBN 9781493936045 (doi: 10.1007/978-1-4939-3606-9_7)

Abstract

The identification of conventional dendritic cells (cDCs) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from other intestinal mononuclear phagocytes such as macrophages (MFs). Here we detail the protocols we have developed to isolate live leukocytes from both murine and human small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs in such preparations.

• Item Type: Book Sections
• Status: Published
• Glasgow Author(s) Enlighten ID: Mowat, Professor Allan and Wright, Miss Pamela and Milling, Professor Simon
• Authors: Scott, C. L., Wright, P. B., Milling, S. W.F., and Mowat, A. M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Publisher: Humana Press
• ISSN: 1064-3745
• ISBN: 9781493936045
• Published Online: 04 May 2016