pVAXhsp65 vaccination primes for high IL-10 production and decreases experimental encephalomyelitis severity

Zorzella-Pezavento, S. F. G. et al. (2017) pVAXhsp65 vaccination primes for high IL-10 production and decreases experimental encephalomyelitis severity. Journal of Immunology Research, 2017, 6257958. (doi: 10.1155/2017/6257958) (PMID:28321419) (PMCID:PMC5339488)

Abstract

Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG35-55) emulsified in Complete Freund’s Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. MOG35-55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction.

• Item Type: Articles
• Additional Information: The study was supported by São Paulo Research Foundation (FAPESP) Grant no. 2011/07528-5 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Grant no. 301770/2009-3.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Camargo da Rosa, Dr Larissa
• Authors: Zorzella-Pezavento, S. F. G., Chiuso-Minicucci, F., França, T. G. D., Ishikawa, L. L. W., da Rosa, L. C., Colavite, P. M., Balbino, B., Marques, C., Ikoma, M. R. V., Masson, A. P., Silva, C. L., and Sartori, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: Journal of Immunology Research
• Publisher: Hindawi
• ISSN: 2314-8861
• ISSN (Online): 2314-7156
• Copyright Holders: Copyright © 2017 Sofia Fernanda Gonçalves Zorzella-Pezavento et al.
• First Published: First published in Journal of Immunology Research 2017: 6257958
• Publisher Policy: Reproduced under a Creative Commons License