Investigation on Microfluidic Particles Manipulation by Holographic 3D Tracking Strategies

Cacace, T., Paturzo, M., Memmolo, P., Vassalli, M. , Fraldi, M., Mensitieri, G. and Ferraro, P. (2017) Investigation on Microfluidic Particles Manipulation by Holographic 3D Tracking Strategies. In: SPIE Optical Metrology, Munich, Germany, 24-27 Jun 2019, (doi: 10.1117/12.2271829)

Abstract

We demonstrate a 3D holographic tracking method to investigate particles motion in a microfluidic channel while unperturbed while inducing their migration through microfluidic manipulation. Digital holography (DH) in microscopy is a full-field, label-free imaging technique able to provide quantitative phase-contrast. The employed 3D tracking method is articulated in steps. First, the displacements along the optical axis are assessed by numerical refocusing criteria. In particular, an automatic refocusing method to recover the particles axial position is implemented employing a contrast-based refocusing criterion. Then, the transverse position of the in-focus object is evaluated through quantitative phase map segmentation methods and centroid-based 2D tracking strategy. The introduction of DH is thus suggested as a powerful approach for control of particles and biological samples manipulation, as well as a possible aid to precise design and implementation of advanced lab-on-chip microfluidic devices.

• Item Type: Conference Proceedings
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Vassalli, Professor Massimo
• Authors: Cacace, T., Paturzo, M., Memmolo, P., Vassalli, M., Fraldi, M., Mensitieri, G., and Ferraro, P.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Proceedings of SPIE - The International Society for Optical Engineering