Holographic detection of nanoparticles using acoustically actuated nanolenses

Ray, A., Khalid, A. , Demcenko, A. , Daloglu, M., Tseng, D., Reboud, J. , Cooper, J. and Ozcan, A. (2020) Holographic detection of nanoparticles using acoustically actuated nanolenses. Nature Communications, 11, 171. (doi: 10.1038/s41467-019-13802-1) (PMID:31949134) (PMCID:PMC6965092)

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The optical detection of nanoparticles, including viruses and bacteria, underpins many of the biological, physical and engineering sciences. However, due to their low inherent scattering, detection of these particles remains challenging, requiring complex instrumentation involving extensive sample preparation methods, especially when sensing is performed in liquid media. Here we present an easy-to-use, high-throughput, label-free and cost-effective method for detecting nanoparticles in low volumes of liquids (25 nL) on a disposable chip, using an acoustically actuated lens-free holographic system. By creating an ultrasonic standing wave in the liquid sample, placed on a low-cost glass chip, we cause deformations in a thin liquid layer (850 nm) containing the target nanoparticles (≥140 nm), resulting in the creation of localized lens-like liquid menisci. We also show that the same acoustic waves, used to create the nanolenses, can mitigate against non-specific, adventitious nanoparticle binding, without the need for complex surface chemistries acting as blocking agents.

Item Type:Articles
Additional Information:The authors acknowledge the support of the NSF Engineering Research Center (ERC, PATHS-UP), NSF CBET, the ARO Life Sciences Division, the Howard Hughes Medical Institute (HHMI), AK acknowledges James Watt, David Brown Mobility and Mac Robertson scholarships. AR acknowledges the Ruth L. Kirschstein Institutional National Research Service Award (5T32DK104687-03) from NIH.
Glasgow Author(s) Enlighten ID:Cooper, Professor Jonathan and Reboud, Professor Julien and Demcenko, Dr Andriejus and Khalid, Mr Arslan
Authors: Ray, A., Khalid, A., Demcenko, A., Daloglu, M., Tseng, D., Reboud, J., Cooper, J., and Ozcan, A.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Nature Communications
Publisher:Nature Research
ISSN (Online):2041-1723
Published Online:16 January 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Nature Communications 11:171
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
168637Advanced Diagnostics using PhononicsJonathan CooperEngineering and Physical Sciences Research Council (EPSRC)EP/K027611/1ENG - Biomedical Engineering
169832Bio-PHONONICS: Advanced Microfluidics & Diagnostics using Acoustic Holograms �` Bio-PhononicsJonathan CooperEuropean Research Council (ERC)340117ENG - Biomedical Engineering