Bussooa, A., Hoare, D., Kirimi, M. T., Mitra, S., Mirzai, N., Neale, S. L. and Mercer, J. (2020) Impedimetric detection and electromediated apoptosis of vascular smooth muscle using microfabricated biosensors for diagnosis and therapeutic treatment in cardiovascular diseases. Advanced Science, 7(18), 1902999. (doi: 10.1002/advs.201902999) (PMID:32999823) (PMCID:PMC7509665)
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Abstract
Cardiovascular diseases remain a significant global burden with 1‐in‐3 of all deaths attributable to the consequences of the disease. The main cause is blocked arteries which often remain undetected. Implantable medical devices (IMDs) such as stents and grafts are often used to reopen vessels but over time these too will re‐block. A vascular biosensor is developed that can report on cellularity and is amenable to being mounted on a stent or graft for remote reporting. Moreover, the device is designed to also receive currents that can induce a controlled form of cell death, apoptosis. A combined diagnostic and therapeutic biosensor would be transformational for the treatment of vascular diseases such as atherosclerosis and central line access. In this work, a cell sensing and cell apoptosing system based on the same interdigitated electrodes (IDEs) is developed. It is shown that the device is scalable and that by miniaturizing the IDEs, the detection sensitivity is increased. Apoptosis of vascular smooth muscle cells is monitored using continuous impedance measurements at a frequency of 10 kHz and rates of cell death are tracked using fluorescent dyes and live cell imaging.
| Item Type: | Articles |
|---|---|
| Additional Information: | This work was supported by the BHF Centre of Research Excellence RE/13/5/30177, University of Glasgow, College of Medical, Veterinary and Life Sciences, Chief Science Office, Scotland Award (CSO): CGA/17/29 and a Medical Research Council Confidence in Concept (MRC-CiC) award. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Hoare, Dr Daniel and Neale, Professor Steven and Bussooa, Anubhav and Mercer, Dr John and Kirimi, Mahmut Talha and Mirzai, Mr Nosrat and Mitra, Dr Srinjoy |
| Authors: | Bussooa, A., Hoare, D., Kirimi, M. T., Mitra, S., Mirzai, N., Neale, S. L., and Mercer, J. |
| College/School: | College of Science and Engineering > School of Engineering College of Science and Engineering > School of Engineering > Systems Power and Energy College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health |
| Journal Name: | Advanced Science |
| Publisher: | Wiley |
| ISSN: | 2198-3844 |
| ISSN (Online): | 2198-3844 |
| Published Online: | 27 July 2020 |
| Copyright Holders: | Copyright © 2020 The Authors |
| First Published: | First published in Advanced Science 7(18):1902999 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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