Tetaud, E., Hall, D.R., Gourley, D.G., Leonard, G.A., Arkison, S. , Barrett, M.P. and Hunter, W.N. (1998) Crystallization and preliminary X-ray diffraction studies of 6-phosphogluconate dehydrogenase from Lactococcus lactis. Acta Crystallographica Section D: Biological Crystallography, 54(6.2), pp. 1422-1424. (doi: 10.1107/s0907444998004909) (PMID:10089526)
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Abstract
6-Phosphogluconate dehydrogenase is one of the seven enzymes involved in the pentose phosphate pathway. Crystals of a mammalian and a protozoan enzyme have been obtained previously and structures determined. It is reported here that a bacterial 6-phosphogluconate dehydrogenase, from Lactococcus lactis, has been purified and used in crystallization trials. Large prisms suitable for a detailed structural analysis have been obtained and characterized as orthorhombic, space group F222, with a = 70.4, b = 105.7, c = 474.6 Å. Diffraction has been observed to 2.2 Å resolution using synchrotron radiation. Structural analysis, in combination with ongoing biochemical characterization, will assist the elucidation of the structure–activity relationships of this enzyme.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Arkison, Mr Scott and Barrett, Professor Michael |
Authors: | Tetaud, E., Hall, D.R., Gourley, D.G., Leonard, G.A., Arkison, S., Barrett, M.P., and Hunter, W.N. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Acta Crystallographica Section D: Biological Crystallography |
Publisher: | International Union of Crystallography |
ISSN: | 0907-4449 |
ISSN (Online): | 1399-0047 |
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