Ludewig, B., Maloy, K. J. , López‐Macías, C., Odermatt, B., Hengartner, H. and Zinkernagel, R. M. (2000) Induction of optimal anti-viral neutralizing B cell responses by dendritic cells requires transport and release of virus particles in secondary lymphoid organs. European Journal of Immunology, 30(1), pp. 185-196. (doi: 10.1002/1521-4141(200001)30:1<185::AID-IMMU185>3.0.CO;2-L) (PMID:10602040)
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Abstract
Dendritic cells (DC) are sentinels of the immune system, transporting antigens from the periphery to secondary lymphoid organs. This study investigates the interactions of DC with B cells for the induction of anti-viral neutralizing antibody responses. Using the vesicular stomatitis virus glycoprotein (VSV-G) as a model antigen, we show that DC contain infection with cytopathic VSV in the presence of a functional IFN system, facilitating transport and release of low levels of live virus in secondary lymphoid organs. DC exposed to live virus induced efficient neutralizing anti-viral B cell responses. In contrast, DC transporting UV-inactivated viral antigens were poor activators of anti-viral B cells, although they were capable of very efficiently inducing virus-specific Th cells. Transgenic DC expressing a membrane-bound form of VSV-G induced neutralizing B cell responses; however, this DC-induced, Th-dependent B cell response was significantly slower than the anti-viral B cell response induced by DC infected with live VSV, and was strongly dependent on concomitant priming of T help. These results suggest that DC may play a double role during infection with cytopathic virus: they transport and release live virus in secondary lymphoid tissues for optimal direct B cell induction and offer MHC class II-associated determinants for induction of T help.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Maloy, Professor Kevin |
| Authors: | Ludewig, B., Maloy, K. J., López‐Macías, C., Odermatt, B., Hengartner, H., and Zinkernagel, R. M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | European Journal of Immunology |
| Publisher: | Wiley |
| ISSN: | 0014-2980 |
| ISSN (Online): | 1521-4141 |
| Published Online: | 30 August 2000 |
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