CYRI/ Fam49 proteins represent a new class of Rac1 interactors

Whitelaw, J. A., Lilla, S., Paul, N. R., Fort, L., Zanivan, S. and Machesky, L. M. (2019) CYRI/ Fam49 proteins represent a new class of Rac1 interactors. Communicative and Integrative Biology, 12(1), pp. 112-118. (doi: 10.1080/19420889.2019.1643665) (PMID:31413787) (PMCID:PMC6682259)

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Fam49 proteins, now referred to as CYRI (CYFIP-related Rac Interactor), are evolutionarily conserved across many phyla. Their closest relative by amino acid sequence is CYFIP, as both proteins contain a domain of unknown function DUF1394. We recently showed that CYRI and the DUF1394 can mediate binding to Rac1 and evidence is building to suggest that CYRI plays important roles in cell migration, chemotaxis and pathogen entry into cells. Here we discuss how CYRI proteins fit into the current framework of the control of actin dynamics by positive and negative feedback loops containing Rac1, the Scar/WAVE Complex, the Arp2/3 Complex and branched actin. We also provide data regarding the interaction between Rac1 and CYRI in an unbiassed mass spectrometry screen for interactors of an active mutant of Rac1.

Item Type:Articles
Additional Information:This work was supported by Cancer Research UK core grant number A17196 and A15673 to LMM, C596/A12935 to SZ. NP is supported by an MRC project grant to LMM (MR/ R017255/1).
Glasgow Author(s) Enlighten ID:Zanivan, Professor Sara
Authors: Whitelaw, J. A., Lilla, S., Paul, N. R., Fort, L., Zanivan, S., and Machesky, L. M.
College/School:College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Journal Name:Communicative and Integrative Biology
Publisher:Landes Bioscience
ISSN (Online):1942-0889
Published Online:17 July 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Communicative and Integrative Biology 12(1):112-118
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
301636Metabolic drivers of pancreatic cancer cell migration and metastasisLaura MacheskyMedical Research Council (MRC)MR/R017255/1CS - Beatson Institute for Cancer Research